ReproSil Saphir 300 CM

Ajánlatkérés
Cikkszám: ra35.cm. (5µm) | ra30.cm. (10µm)
Gyártó: Dr. Maisch

Leírás és Paraméterek

Wide-Pore Weak Cation Exchange Column for Large Biomolecule Separations

ReproSil Saphir 300 CM from Dr. Maisch HPLC GmbH is a high-purity silica-based HPLC column with a 300 Å wide-pore structure and a carboxymethyl weak cation exchange bonded phase. This stationary phase offers controlled ionic interactions for positively charged analytes, making it ideal for the separation of proteins, peptides, and other biomolecules with basic functional groups. The wide-pore design ensures excellent accessibility and efficient mass transfer for high-molecular-weight analytes, while the CM functionality provides milder ion-exchange conditions compared to strong cation exchangers, preserving biomolecule structure and activity.

ReproSil Saphir 300 CM columns from Dr. Maisch HPLC GmbH combine wide-pore accessibility with gentle ion-exchange selectivity – the ideal choice for sensitive large biomolecule HPLC separations.

Specifications

Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification Carboxymethyl
Particle Type
Separation Mode
USP Code
Endcapping No
Carbonload 3 %
pH Range 2 to 8

Key Features:

  • Fully porous, ultra-pure silica with 300 Å pore size

  • Weak cation exchange bonded phase for gentle protein separations

  • Optimized for large biomolecules including proteins and peptides

  • High resolution, reproducibility, and peak symmetry

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm and 10 µm particle sizes

Typical Applications:

  • Protein and peptide separations under mild ion-exchange conditions

  • Biopharmaceutical QC and characterization of basic biomolecules

  • Fractionation of protein isoforms and charge variants

  • Method development for biomolecule purification