ReproSil Saphir
Ultra-Pure Silica HPLC Columns for Superior Reversed-Phase Performance
ReproSil Saphir is a high-end HPLC column series from Dr. Maisch HPLC GmbH, based on ultra-pure, fully porous spherical silica and designed for demanding reversed-phase applications. With advanced C18 bonding and optimized endcapping, ReproSil Saphir columns provide excellent peak shape, low silanol activity, and high separation efficiency — ideal for basic compounds, closely eluting analytes, and method validation.
These columns are particularly suited for use in pharmaceutical quality control, impurity profiling, and regulatory-compliant workflows, where precision and reproducibility are paramount.
ReproSil Saphir columns from Dr. Maisch HPLC GmbH offer premium reversed-phase performance – the ideal choice for laboratories requiring maximum selectivity, reproducibility, and efficiency.
Specifications
| Particle Sizes | 1.5 – 15 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, Carboxymethyl, NH2, Propyl-SAX, SCX, Si |
| Pore Sizes | 100 Å, 300 Å, 1 000 Å |
| Surface Areas | 30 m²/g, 100 m²/g, 400 m²/g |
Key Features:
-
Ultra-pure spherical silica with optimized C18 surface chemistry
-
Excellent peak symmetry, even for basic and polar compounds
-
High column efficiency and reproducibility
-
Suitable for gradient and isocratic reversed-phase HPLC
-
Manufactured under strict quality standards by Dr. Maisch HPLC GmbH
Typical Applications:
-
Pharmaceutical impurity profiling and stability testing
-
Method development and validation in regulated environments
-
Analysis of basic drugs and polar analytes
-
Reversed-phase separations requiring sharp peak resolution
Products
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil Saphir 100 C4 | 5 – 10 µm | 8 % | 100 Å |
| ReproSil Saphir 100 C8 | 5 – 10 µm | 12 % | 100 Å |
| ReproSil Saphir 100 C18 | 1.5 – 10 µm | 20 % | 100 Å |
| ReproSil Saphir 100 NH2 | 5 µm | 4 % | 100 Å |
| ReproSil Saphir 100 SAX | 5 µm | 6 % | 100 Å |
| ReproSil Saphir 100 SCX | 3 – 10 µm | 6 % | 100 Å |
| ReproSil Saphir 300 CM | 5 – 10 µm | 3 % | 300 Å |
| ReproSil Saphir 300 SCX | 5 – 15 µm | 6 % | 300 Å |
| ReproSil Saphir 1000 Si | 10 µm | 0 % | 1 000 Å |
| ReproSil Saphir 1000 C18-2 HE | 7 – 10 µm | 3 % | 1 000 Å |
ReproSil Saphir 100 C18 (direct replacement for Luna C18)
| ReproSil Saphir 100 C18, 1.5 µm | ra115.9e. |
|---|---|
| ReproSil Saphir 100 C18, 1.8 µm | ra118.9e. |
| ReproSil Saphir 100 C18, 2.3 µm | ra122.9e. |
| ReproSil Saphir 100 C18, 3 µm | ra13.9e. |
| ReproSil Saphir 100 C18, 3.5 µm | ra135.9e. |
| ReproSil Saphir 100 C18, 5 µm | ra15.9e. |
| ReproSil Saphir 100 C18, 10 µm | ra10.9e. |
ReproSil Saphir 100 C8 (direct replacement for Luna C8)
ReproSil Saphir 1000 Si
Extra-Wide-Pore Unmodified Silica Column for Large Biomolecule Chromatography
ReproSil Saphir 1000 Si from Dr. Maisch HPLC GmbH is a high-purity, fully porous silica HPLC column with an extra-wide 1000 Å pore size, designed for the separation of very large biomolecules and macromolecular complexes. Without any bonded phase modification, the unmodified silica surface provides highly polar interaction properties, making it suitable for normal-phase chromatography and specific bioanalytical applications. The extremely large pores allow optimal accessibility and efficient mass transfer for analytes such as monoclonal antibodies, protein complexes, viruses, and nanoparticles.
ReproSil Saphir 1000 Si columns from Dr. Maisch HPLC GmbH deliver exceptional accessibility and polar surface selectivity – the ideal choice for very large biomolecule and specialized normal-phase HPLC applications.
Specifications
| Particle Size |
|
|---|---|
| Pore Size | 1 000 Å |
| Surface Area | 30 m²/g |
| Phase/Modification | Si |
| Particle Type | |
| Separation Mode | |
| Endcapping | No |
| Carbonload | 0 % |
| pH Range | 2 to 8 |
Key Features:
-
Fully porous, ultra-pure unmodified silica with 1000 Å pore size
-
Ideal for very large biomolecules and macromolecular assemblies
-
Suitable for normal-phase and specialized bioanalytical applications
-
Excellent mass transfer and accessibility for high-MW analytes
-
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
-
Available in 10 µm
Typical Applications:
-
Size-based separations of large biomolecules
-
Analysis of monoclonal antibodies (mAbs) and protein complexes
-
Normal-phase chromatography of polar large-molecule analytes
-
Biopharmaceutical QC and preparative purification
ReproSil Saphir 300 SCX