ReproSil Saphir 1000 Si

Ajánlatkérés
Cikkszám: ra.60.00 (10µm)
Gyártó: Dr. Maisch

Extra-Wide-Pore Unmodified Silica Column for Large Biomolecule Chromatography

ReproSil Saphir 1000 Si from Dr. Maisch HPLC GmbH is a high-purity, fully porous silica HPLC column with an extra-wide 1000 Å pore size, designed for the separation of very large biomolecules and macromolecular complexes. Without any bonded phase modification, the unmodified silica surface provides highly polar interaction properties, making it suitable for normal-phase chromatography and specific bioanalytical applications. The extremely large pores allow optimal accessibility and efficient mass transfer for analytes such as monoclonal antibodies, protein complexes, viruses, and nanoparticles.

ReproSil Saphir 1000 Si columns from Dr. Maisch HPLC GmbH deliver exceptional accessibility and polar surface selectivity – the ideal choice for very large biomolecule and specialized normal-phase HPLC applications.

Specifications

Particle Size
  • 10 µm
Pore Size 1 000 Å
Surface Area 30 m²/g
Phase/Modification Si
Particle Type
Separation Mode
Endcapping No
Carbonload 0 %
pH Range 2 to 8

Key Features:

  • Fully porous, ultra-pure unmodified silica with 1000 Å pore size

  • Ideal for very large biomolecules and macromolecular assemblies

  • Suitable for normal-phase and specialized bioanalytical applications

  • Excellent mass transfer and accessibility for high-MW analytes

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 10 µm

Typical Applications:

  • Size-based separations of large biomolecules

  • Analysis of monoclonal antibodies (mAbs) and protein complexes

  • Normal-phase chromatography of polar large-molecule analytes

  • Biopharmaceutical QC and preparative purification

Leírás és Paraméterek

Wide-Pore Strong Cation Exchange Column for Large Biomolecule Separations

ReproSil Saphir 300 SCX from Dr. Maisch HPLC GmbH is a high-purity silica-based HPLC column with a 300 Å wide-pore structure and a strong cation exchange bonded phase based on sulfonic acid functional groups. This phase provides strong ionic retention for positively charged analytes, delivering high resolution, sharp peak shapes, and reproducible retention times. The wide-pore design ensures optimal accessibility and efficient mass transfer for high-molecular-weight analytes such as proteins, enzymes, and peptides, making it particularly suitable for biopharmaceutical and proteomics applications.

ReproSil Saphir 300 SCX columns from Dr. Maisch HPLC GmbH combine wide-pore accessibility with strong ionic selectivity – the optimal choice for high-resolution large biomolecule chromatography.

Specifications

Particle Sizes
  • 5 µm
  • 10 µm
  • 15 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification SCX
Particle Type
Separation Mode
USP Codes
Endcapping No
Carbonload 6 %
pH Range 2 to 8

Key Features:

  • Fully porous, ultra-pure silica with 300 Å pore size

  • Strong cation exchange (SCX) phase with sulfonic acid groups

  • High selectivity for basic biomolecules and peptides

  • Excellent reproducibility, resolution, and peak symmetry

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm, 10 µm, and 15 µm particle sizes

Typical Applications:

  • Protein and peptide separations via strong cation exchange

  • Biopharmaceutical QC and charge variant analysis

  • Fractionation of protein isoforms in proteomics

  • Preparative purification of basic biomolecules